Figure 1. E. coli BL21 (DE3) cells with no DNA control, after 24 h of incubation at 37 °C.
Figure 2. E. coli BL21 (DE3) colonies after 24 h of incubation at 37 °C. Error in plates: agar plate used.
Figure 3. Incubation of cells, after 24 h at 37 °C, from cell phone swabs; fun plate. Error in plate: agar plate was not used.
Figure 4. E. coli BL21 (DE3) colonies after 24 h of incubation at 37 °C. Cell plate of Neusha and Carolyn due to original having error.
Figure 5. Erlenmeyer flask of pGEM-gbr22 protein in a solution of ampicillin and LB broth.
Figure 6. E. coli expressing pGEM-gbr22 protein after centrifuging of the sample and removal of the resulting supernatant liquid.
Figure 7. 15 mL conical tubes containing elution one (bottom), which comprises most of the expressed protein, after being run through a Ni-NTA column; elution two (top) contains that which remains after elution one is collected, and the Ni-NTA column is washed through again.
Figure 8: SDS gel electrophoresis done at 200V for 25 minutes after staining. The furthest column on the left represents a ladder. This is followed by Samples one through six collected from previous protein labs. Purple bands represent protein found in the sample. The intensity of color represents the amount of the protein in that specific sample. The position of the purple band compared to the molecular weights standard represents the molecular weight of the protein in kDa. ~25kDa.
Introduction:
Materials & Methods:
Results:
Discussion:
Conclusions:
References: